Composite

Part:BBa_K1085040:Design

Designed by: Mirjam Groenewold   Group: iGEM13_Groningen   (2013-09-17)

MotB Strep-tag SilkSubunitN1 SilkSubunitN2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was codon-optimized for Bacillus subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.


Source

The amino acids sequence of the silk protein was obtained from MaSp2 of the Argiope aurantia. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).


References